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A subnet mask marks network bits with 1s and host bits with 0s (e.g. 255.255.255.0). A Cisco ACL wildcard mask is its inverse (0.0.0.255) — 0 bits must match, 1 bits are ignored. The Wildcard / ACL tab converts between them automatically.
Variable Length Subnet Masking lets you carve subnets of different sizes from one address block instead of wasting addresses on equal-sized subnets. Getting a boundary wrong causes two subnets to overlap, which the VLSM Planner and Compare tabs both catch.